Molecular Cloning and Physical Mapping of the Genome of Bombyx Mori Nuclear Polyhedrosis Virus
نویسندگان
چکیده
منابع مشابه
Molecular cloning and physical mapping of the genome of Bombyx mori nuclear polyhedrosis virus.
A restriction fragment library which covered the entire genome of Bombyx mori nuclear polyhedrosis virus (BmNPV) was constructed using plasmid vectors. By analysis of cloned and viral DNA by double digestion with endonucleases and by hybridization techniques, a complete physical map of BmNPV was constructed for BamHI, EcoRI, HindIII, KpnI, PstI and SmaI. Five regions of repeated sequences conta...
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Two structurally different classes of oligonucleotides accumulate in vitro in cytoplasmic polyhedrosis virus (CPV) transcription mixtures in molar excess as compared to the completed RNA products. The first class consists of oligonucleotides which correspond to the 5’terminal sequence of the virus mRNAs (referred to as initiator oligonucleotides). The major species of initiator oligonucleotides...
متن کاملMolecular characterization of Bombyx mori cytoplasmic polyhedrosis virus genome segment 4.
The complete nucleotide sequence of the genome segment 4 (S4) of Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) was determined. The 3,259-nucleotide sequence contains a single long open reading frame which spans nucleotides 14 to 3187 and which is predicted to encode a protein with a molecular mass of about 130 kDa. Western blot analysis showed that S4 encodes BmCPV protein VP3, which is on...
متن کاملThe p95 gene of Bombyx mori nuclear polyhedrosis virus: temporal expression and functional properties.
As part of our effort to identify baculovirus proteins acting as transcriptional regulators, we have characterized a gene, p95, of Bombyx mori nuclear polyhedrosis virus (BmNPV) that encompasses an open reading frame for a putative 95-kDa polypeptide (P95). The N-terminal half of the conceptually translated P95 contains two zinc finger-type DNA-binding motifs, and its C terminus contains a prol...
متن کاملDivergence and evolution of homologous regions of Bombyx mori nuclear polyhedrosis virus.
Homologous regions (hrs) (hr1,hr2-left,hr2-right,hr3,hr4-left,hr 4-right, and hr5) similar to those found in the Autographa californica nuclear polyhedrosis virus (AcNPV) genome were found in the Bombyx mori NPV (BmNPV) genome. The BmNPV hrs contained two to eight repeats of a homologous nucleotide sequence which were on average about 75 bp long. All of these homologous sequence repeats contain...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1990
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-71-8-1851